The Shift of ERG B-Wave Induced by Hours' Dark Exposure in Rodents

نویسندگان

  • Dake Li
  • Qi Fang
  • Hongbo Yu
چکیده

PURPOSE Dark adaptation can induce a rapid functional shift in the retina, and after that, the retinal function is believed to remain stable during the continuous dark exposure. However, we found that electroretinograms (ERG) b-waves gradually shifted during 24 hours' dark exposure in rodents. Detailed experiments were designed to explore this non-classical dark adaptation. METHODS In vivo ERG recording in adult and developing rodents after light manipulations. RESULTS We revealed a five-fold decrease in ERG b-waves in adult rats that were dark exposed for 24 hours. The ERG b-waves significantly increased within the first hour's dark exposure, but after that decreased continuously and finally attained steady state after 1 day's dark exposure. After 3 repetitive, 10 minutes' light exposure, the dark exposed rats fully recovered. This recovery effect was eye-specific, and light exposure to one eye could not restore the ERGs in the non-exposed eye. The prolonged dark exposure-induced functional shift was also reflected in the down-regulation on the amplitude of intensity-ERG response curve, but the dynamic range of the responsive light intensity remained largely stable. Furthermore, the ERG b-wave shifts occurred in and beyond classical critical period, and in both rats and mice. Importantly, when ERG b-wave greatly shifted, the amplitude of ERG a-wave did not change significantly after the prolonged dark exposure. CONCLUSIONS This rapid age-independent ERG change demonstrates a generally existing functional shift in the retina, which is at the entry level of visual system.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

PREVENTIVE EFFECTS OF VITAMIN B12 AND FOLIC ACID IN METHANOL-INDUCED RETINOPATHY IN AN ANIMAL MODEL

In order to identify the effects of vitamin B 12 and folic acid in reducing methanolinduced retinopathy, ERG waves were compared before and after methanol intoxication in rabbits. In this study 34 rabbits were divided into 4 groups. All groups received 4 g/kg of methanol intraperitoneally. In addition, the second, third and fourth groups received vitamin B 12 injection, oral folic acid and ...

متن کامل

PREVENTIVE EFFECTS OF VITAMIN B12 AND FOLIC ACID IN METHANOL-INDUCED RETINOPATHY IN AN ANIM AL MODEL

In order to identify the effects of vitamin B 12 and folic acid in reducing methanolinduced retinopathy, ERG waves were compared before and after methanol intoxication in rabbits. In this study 34 rabbits were divided into 4 groups. All groups received 4 g/kg of methanol intraperitoneally. In addition, the second, third and fourth groups received vitamin B 12 injection, oral folic acid and ...

متن کامل

A circadian clock regulates the process of ERG b- and d-wave dominance transition in dark-adapted zebrafish

In zebrafish, during dark adaptation following bright light adaptation, the dominance of electroretinogram (ERG) b- and d-waves switches. In the early dark adaptation, when visual sensitivity is cone-dominant, both the b- and d-waves are readily recorded. In the late dark adaptation, along with the increase of rod sensitivity, the b-wave becomes dominant whereas the d-wave is gradually lost. Th...

متن کامل

ERG dark adaptation in the unanesthetized rabbit.

The amplitude of the rabbit scotopic electroretinogram (ERG) is notoriously labile both in day-to-day variations in the same animal1 as well as in the wide range seen in normal controls. This seriously impairs the rabbit's usefulness as a model in long-term experiments. Part of the reason for this difficulty appears to lie in the traditional methods of obtaining and assessing adaptation prepara...

متن کامل

Dark adaptation in locally detached retina.

Nonrhegmatogenous retinal detachments were formed in the eyes of Dutch rabbits by subretinal injection of Hanks' balanced salt solution. The electroretinogram (ERG) was recorded locally from the acutely detached retina, and simultaneously from the surrounding attached retina (vitreal ERG [VERG]), before and after exposure to diffuse intense irradiation. Light adaptation elevated b-wave threshol...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 11  شماره 

صفحات  -

تاریخ انتشار 2016